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KMID : 0921620070370040213
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Journal of Bacteriology and Virology
2007 Volume.37 No. 4 p.213 ~ p.224
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Comparison of Proteome Component of Helicobacter pylori in Different Atmospheric CO2 Concentration
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Lee Seung-Gyu
Kim Kyung-Mi
Park Jeong-Won
Kwon Young-Chul
Song Jae-Young
Youn Hee-Shang
Seo Ji-Hyun
Baik Seung-Chul
Lee Woo-Kon
Cho Myung-Je
Rhee Kwang-Ho
Kang Hyung-Lyun
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Abstract
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Helicobacter pylori is a spiral, slow growing gram-negative microaerophilic bacterium. It has been shown to be the etiological agent of gastroduodenal diseases, such as chronic gastritis, gastric and duodenal ulcers, and gastric cancer. General culture condition of H. pylori is 5% O2, 10% CO2 and 100% humid atmosphere. We have compared proliferation protein expression profile of H. pylori incubated under normal microaerophilic (10% CO2) and environment stress (4% CO2, 18% CO2) conditions. H. pylori cultured under environment stress displayed coccoid morphology and timedependent decrease in proliferation. We have further compared the protein expression profiles of H. pylori under normal growing and environment stress conditions by a global proteomic analysis, which includes high-resolution 2-DE followed by matrix-assisted laser desorption/ionization time of flight and nanoelectrospray/tandem mass spectrometry. In total, 42 protein spots were found to be up- or down-regulated by more than 2-fold under environment stress conditions. Of the 42 protein spots processed, 27 spots were identified; they represented 19 genes, including 2 kinds of hypothetical proteins.
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KEYWORD
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Helicobacter pylori, Proteome, Environment stress
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